Threshold concepts are concepts that when mastered, represent a transformed understanding of a discipline, without which the learner cannot progress. They are therefore essential for learning. By focusing on threshold concepts, instructors can maximize the impact of instruction. During this workshop, participants will be introduced to what is known about threshold concepts in biochemistry and how these concepts relate to prerequisite coursework. Workshop participants will contribute to a community-based effort to create instructional and assessment tools to support undergraduate students in gaining a deeper understanding of selected biochemical threshold concepts. Participation in this process will provide faculty the resources and skills needed to begin to change their instruction to better address learning of these vital concepts. Those who teach undergraduate biochemistry as well as those who teach prerequisite courses for biochemistry (general chemistry, organic chemistry, general biology) are encouraged to attend the workshop.